Peptide · Research Monograph · 43-amino acid fragment of Thymosin Beta-4 (Tβ4) active region

TB-500

Cell Migration Studies

Also known as TB500 · Thymosin Beta-4 fragment

TB-500 is a fragment of thymosin beta-4, a protein found in most human cell types. The fragment reproduces the section that binds actin, the protein that gives cells their shape and allows them to move. Studies have measured how cells migrate in culture and how new blood vessels form under those conditions.

Formula
C212H350N56O78S
Mol. weight
4963.44 Da
Length
43 residues
Size
10mg
View product & pricing → Lot records & COAs

For laboratory research use only - not for human or animal use

TB-500 vial from the Eon Peptides research catalog
TB-500 · 10mg · catalog photograph
Available in the Eon catalog - TB-500 from $85.00 View certificate of analysisAccurate Test Labs · 99.26% purity · Lot TB5-090226-10

The molecule, in three dimensions

C212H350N56O78S4963.44
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Residues · hover to locate43 residues
Heavy atoms
347
Bonds
350
Composition
C212 N56 O78 S1
Rings
4
Metal centre
None
Chain length
43 residues

Every heavy atom is placed from the sequence with ideal bond lengths and angles, so the connectivity is exact. The pose is idealised, an ideal helix, and is not a measured conformation. Identity for the lot in stock is confirmed by LC-MS on its certificate.

Structure viewer →

Molecular data

Molecular formulaC212H350N56O78S
Molecular weight4963.44 Da
Sequence length43 residues
Physical formLyophilized Powder
Available sizes10mg

How it works

  1. Actin Binding

    Motility and Migration of Cells

    The mechanism characterised most often is G-actin monomer binding by TB-500. Preclinical models then measure migration of cells toward injury sites and the polymerisation of actin.

    • G-actin sequestering regulates polymerization
    • Cells measured moving toward injury sites
    • Cytoskeleton remodelling was observed
  2. Cardiac Research

    ILK Signalling in Cardiac Models

    Cardiac studies of TB-500 examine cardiac progenitor cell migration and integrin-linked kinase (ILK) signalling.

    • Integrin-linked kinase (ILK) is activated
    • Cardiac progenitor migration is quantified
    • Progenitor migration models in cardiac tissue
  3. Cytokine Profile

    Modulation of Inflammatory Signalling

    Preclinical wound models record changed macrophage activity and reduced pro-inflammatory cytokines, the immune profile moving away from inflammatory markers toward those of the reparative phase.

    • Measured reductions in pro-inflammatory cytokines
    • Macrophage polarization is modulated
    • Observed tissue remodelling of an anti-fibrotic character

What the research shows

  1. Dermal Research

    Models of Wound Closure

    Preclinical wound models examine organized collagen deposition, scarring and closure of dermal wounds.

  2. Cardiac Research

    Heart Tissue Models

    Post-infarction models examine migration of cardiac cells driven by ILK activation.

  3. Ocular Research

    Models of the Cornea

    Ocular surface models examine inflammation and the closure of corneal epithelial wounds.

  4. Soft Tissue

    Muscle and Tendon Models

    Ligament, tendon and muscle models examine fibrotic scoring and cell migration mediated by actin.

How it's tested

Every lot of TB-500 released to the catalog carries a third-party certificate of analysis. 5 documented lots are on file for this compound, with reported HPLC purity from 99.17% to 99.86% (mean 99.52%), issued by Accurate Test Labs and Freedom Diagnostics. 4 of 5 certificates record identity as confirmed.

Documented lots
5
Reported purity
99.17% to 99.86%
Mean purity
99.52%
Issuing labs
Accurate Test Labs, Freedom Diagnostics

All certificates in the COA library →

Frequently asked questions

How is TB-500 classified and what is its structure?

TB-500 is a synthetic peptide matching Thymosin Beta-4 (Tβ4) across its active region. Tβ4 is a protein of 43 amino acids occurring naturally in every nucleated cell. Through interaction with the actin cytoskeleton, Tβ4 is investigated for roles in tissue remodelling, differentiation and cell migration.

What distinguishes TB-500 structurally from full-length Thymosin Beta-4?

Tβ4 is the entire sequence of 43 amino acids, whereas TB-500 carries its active fragment, the region that accounts for cell migration properties and actin binding. The core mechanism of action is the same for both.

What regulatory standing does TB-500 hold?

Neither the FDA nor any other regulatory agency has approved TB-500 for human use. As a thymosin beta-4 fragment it is provided solely for laboratory research in vitro, and for the material supplied here a human safety profile has not been established.

Which research fields make use of TB-500?

Preclinical models of inflammatory signalling, tendon and muscle, the corneal surface, cardiac tissue and wound closure all feature TB-500. One primary mechanism runs through every one of these fields: actin regulation leading to cell migration.

What storage conditions preserve lyophilized TB-500?

Long-term stability of lyophilized TB-500 calls for -20°C. Light exposure and repeated freeze-thaw cycling are to be avoided.

Does TB-500 correspond to a pharmaceutical thymosin beta-4 formulation?

No. Regulatory approval has not been granted to TB-500, and the FDA has not approved it for any indication. The material supplied here differs from the pharmaceutical formulation of thymosin beta-4 and is offered solely for laboratory research in vitro.

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For laboratory research use only. Not a drug, supplement, or medical product; not for human or animal use. All findings referenced are from published preclinical/laboratory research.