Peptide · Research Monograph · EPO helix-B surface derived 11-amino-acid peptide

ARA-290

EPO-derived 11-mer · innate repair receptor agonist

ARA-290 is also called cibinetide. It is a peptide made of 11 amino acids, taken from one section of erythropoietin. That is the hormone that drives red blood cell production. ARA-290 was designed to keep signalling at the innate repair receptor while leaving out the red blood cell effect. Research has measured its activity at that receptor, a two-part complex, and examined nerve and inflammation endpoints.

Formula
C55H89N19O20
Mol. weight
1257.3 Da
Length
11 residues
CAS
1208243-50-8

For laboratory research use only - not for human or animal use

ARA-290 vial from the Eon Peptides research catalog
ARA-290 · 10mg · catalog photograph
Available in the Eon catalog - ARA-290 from $60.00

The molecule, in three dimensions

C55H89N19O201257.3
Drag to turn
Residues · hover to locate11 residues
Heavy atoms
88
Bonds
88
Composition
C51 N16 O21
Rings
1
Metal centre
None
Chain length
11 residues

Every heavy atom is placed from the sequence with ideal bond lengths and angles, so the connectivity is exact. The pose is idealised, an extended chain, and is not a measured conformation. Identity for the lot in stock is confirmed by LC-MS on its certificate.

Molecular data

Molecular formulaC55H89N19O20
Molecular weight1257.3 Da
SequencepGlu-Glu-Gln-Leu-Glu-Arg-Ala-Leu-Asn-Ser-Ser
Sequence length11 residues
CAS / identifier1208243-50-8
Physical formLyophilized powder
Available sizes10mg

How it works

  1. IRR Target

    Agonist at the EPOR/CD131 Repair Receptor

    The helix-B surface of erythropoietin (EPO) was the template for ARA-290 (cibinetide), a peptide of 11 amino acids. It targets the innate repair receptor (IRR), a heterocomplex of the EPO receptor with the β-common receptor (CD131). Activation is selective: the classical receptor responsible for red-cell production is not engaged.

    • Agonist selective for the IRR (EPOR/βcR)
    • Hematocrit is unaffected - the peptide is non-erythropoietic
    • Origin is the helix-B surface domain of EPO
  2. Inflammation

    Inflammatory Resolution

    Signalling via the innate repair receptor has been tied to measured falls in pro-inflammatory signalling and to resolution markers. The same models also showed less collateral damage to tissue.

    • Signaling by pro-inflammatory cytokines is suppressed
    • Macrophages move toward a phenotype associated with repair
    • In pain models, activation of spinal microglia is suppressed
  3. Cell Survival

    Neural and Cytoprotective Signalling

    An activated IRR engages the JAK2/STAT3 and PI3K/Akt pathways plus anti-apoptotic pathways of the Bcl-2 family. Although the plasma half-life of the peptide is very short, cell survival and small-fibre measures have been recorded.

    • Survival signaling through JAK2/STAT3 and PI3K/Akt is activated
    • Engages the anti-apoptotic Bcl-2 family
    • Research models have measured small-fibre density

What the research shows

  1. Nerve Pain Models

    Small-Fiber Neuropathy in Sarcoidosis Research

    Models of small-fiber neuropathy are one setting where ARA 290 is studied. Sarcoidosis research has used physical-function measures and neuropathic symptom scores.

  2. Nerve Fibres

    Nerve Fibre Density in Cornea and Skin

    Small-fibre measures, corneal nerve fibre density among them, were recorded together with neuropathic symptom scores across an exposure period of 28 days.

  3. Rodent Pain Work

    Allodynia Thresholds Over the Long Term

    Rodent model studies tracked cold and mechanical allodynia thresholds across 20 weeks, with spinal microglia response in parallel. A relationship that depended on exposure was reported.

  4. Peptide Engineering

    Cytoprotection Derived from EPO

    The tertiary structure of EPO was the starting point for engineering ARA 290. The aim was selective engagement of the innate repair receptor while omitting the erythropoietic activity that native EPO has.

How it's tested

Every lot of ARA-290 released to the catalog carries a third-party certificate of analysis. 1 documented lot is on file for this compound, with reported HPLC purity from 99.89% to 99.89% (mean 99.89%), issued by Freedom Diagnostics.

Documented lots
1
Reported purity
99.89% to 99.89%
Mean purity
99.89%
Issuing labs
Freedom Diagnostics

All certificates in the COA library →

Frequently asked questions

How is ARA-290 classified and what is its structural origin?

ARA-290, also named cibinetide, is a synthetic peptide 11 amino acids long whose design is taken from the helix-B surface domain of erythropoietin (EPO). The intent was selective engagement of the innate repair receptor, leaving out the activity of native EPO that stimulates red blood cells.

How is the innate repair receptor (IRR) defined?

The IRR joins the EPO receptor and the β-common receptor (CD131) as a heterocomplex. Erythropoiesis is controlled by the classical homodimeric EPO receptor, and the IRR is a separate arm of EPO signalling. ARA-290 activates the IRR selectively.

How does ARA-290 differ from EPO with respect to erythropoiesis?

ARA-290 is non-erythropoietic. The receptor pathway behind red-cell production is not engaged by the peptide, and hematocrit therefore does not rise. Separating those two receptors is the core rationale of the design.

In which research models has ARA 290 been examined?

Small-fiber neuropathy models are the main setting. Measures examined there include regrowth of small nerve fibers, corneal nerve fiber density and neuropathic symptom scores. Long-term neuropathic pain behaviors are also examined in preclinical models.

What is the intended-use status of research-grade ARA-290?

Research-grade ARA-290 is strictly for analytical applications and in vitro laboratory research. It is not a drug. It is not intended for any clinical application, nor for human or veterinary use.

For laboratory research use only. Not a drug, supplement, or medical product; not for human or animal use. All findings referenced are from published preclinical/laboratory research.